A 2-phase liquid scintillation assay for glycolipid synthetases
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چکیده
منابع مشابه
A 2-Phase Liquid Scintillation Assay for Glycolipid Synthetases
Glycolipid synthetases can be assayed conveniently by incubating the lipid substrate with the radiosugar-labeled nucleotide in a small plastic scintillation vial. At the end of the incubation period, water and perchloric acid are added, then n-butanol, then a toluene-based scintillation cocktail. The radioactive lipid partitions into the scintillation fluid, leaving excess sugar nucleotide in t...
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ژورنال
عنوان ژورنال: Lipids
سال: 1981
ISSN: 0024-4201,1558-9307
DOI: 10.1007/bf02535347